Petra Matjan Štefin

CORE QUALIFICATIONS

Laboratory animals; cancer research; cancer biology; tumor microenvironment; tumor markers; tumor cell culture; in vivo imaging; histology; immunohistochemistry; immunofluorescence

EXPERIENCE
    • April, 2019 – present LABORATORY ANIMAL VETERINARIAN, “Jožef Stefan” Institute, Department of Biochemistry, Molecular and Structural Biology
    • October, 2019 – present YOUNG RESEARCHER,  PhD STUDENT   “Jožef stefan” Institute, Department of Biochemistry, Molecular and Structural Biology
EDUCATION

2011 – 2018 Veterinary Faculty, University of Ljubljana

  • Doctor of Veterinary Medicine
  • Veterinary Faculty Prešeren Award for the research assignment Incidence and Clinicopathological Characteristics of Lesions in the Rabbits’ Female Genital System; December 2016.

 

 

Georgy Mikhaylov

Education
2014, September PhD in Nanoscience and Nanotechnology. Jozef Stefan International Postgraduate School, Ljubljana, Slovenia
2007, June M.Sc. in Clinical Biochemistry. Faculty of Medical Biology, Siberian State Medical University, Tomsk, Russia (Graduated summa cum laude)
2001, January M.Sc. in Economy. Faculty of Business Administration, University of International Business, Almaty, Kazakhstan
Research and Professional Experience
2018-presence Researcher, Junior Team leader

Current research interests: in vivo Imaging, Metabolic Biochemistry, Protease Biology, Targeted Drug Delivery for Diagnostic and Therapeutic Applications.

2015-2018

Laboratory of Bioorganic Chemistry and Molecular Imaging, Federal School of Technology, Lausanne, Switzerland (EPFL).

Postdoctoral Researcher

Mentor: Tenure Track Assistant Professor Elena Goun (Dubikovskaya)

Achievements: New bioluminescent probes for monitoring of biological processes in real-time in a living system using biocompatible chemical reactions have been developed. Probes demonstrated excellent selectivity and a high potential for visualization of metabolic uptake of biological molecules (glucose, nicotinamide riboside) and detection of proteolytic activity in pathological conditions both in vitro and in vivo.

Research outputs: This work was presented at several international conferences and published in Nature Methods journal (IF 28.467).

2014–2015

Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Ljubljana, Slovenia

Postdoctoral Researcher, Head of the Histology Laboratory

Mentor: Associate Professor Olga Vasiljeva

Achievements:

The study demonstrated that nanoparticle-based systems capable of disturbing extracellular tumor microenvieronment ion balance is a novel class of inorganic materials that exhibit a strong antitumor effect.

Research outputs: this work was published in NanoLetters journal (IF 12.080) and possible application of investigated nanomaterials was covered in the patent application.

2008-2014

Jozef Stefan International Postgraduate School, Ljubljana, Slovenia

PhD student, Research Assistant

Mentor: Associate Professor Olga Vasiljeva

Thesis: Development of a Novel Nano-Carriers for Targeted Drug Delivery and Diagnostics

Achievements: New delivery platforms for targeting the tumor and its microenvironment, based on ferrimagnetic nano-clusters (ferri-liposomes) and lipidated inhibitor of cysteine protease, cathepsin B, (LNC-NS-629), have been developed. Ferri-liposomes and LNC-NS-629 were shown to act as universal drug delivery systems, confirmed by the successful delivery of several chemically different types of cargo (therapeutic and diagnostic) to the tumor site.

Research outputs: This work was presented at several international conferences including two oral presentations at XI International Conference on Nanostructured Materials (Rhodes, Greece) and Gordon Research Conference (Barga, Italy). This work was published in Nature Nanothechnology Journal (IF 33.265), Angewandte Chemie International Edition Journal (IF 11.336). Development and possible applications of the developed targeting systems were covered in two patent applications.

2007-2008

Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Ljubljana, Slovenia

Research Associate

Achievements: demonstrated the possible use of Fe3O4 nanoparticles as a MRI visible contrast agents for cancer diagnostics.

Research outputs: this work was presented at an international conference and one review publication.

2001-2007

Department of Human Morphology, Siberian State Medical University, Tomsk, Russia

Master student

Mentor: Professor Irina Sukhodolo

Thesis: An assessment of acute toxicity of Fe3O4 nanoparticles in rats.

Achievements: The possible adverse effects of Fe3O4 nanoparticles were evaluated in acute experiments on rats. The investigated nanoparticels were nontoxic in high doses demonstrating the possible use of them in targeted drug delivery systems.

Research outputs: this work resulted in one publication.

   
Publications
2019 Maric T1, Mikhaylov G1, Khidakivskyi P, Bazhin A, Sinisi R, Bonhoure N, Yevtodiyenko, Jones A, Muhunthan V, Abdelhady G, Shackelford D, Goun E. Biolumenescent-based imaging and quantification of glucose uptake in vivo. Nature Methods. 2019. 16(6):526-532. (impact factor 28.467).
2019 Bratos A, Kramer L, Mikhaylov G, Vasiljeva O, Turk B. Stefin A-functionalized liposomes as a system for cathepsin S and L-targeted drug delivery. Biochimie. 2019. pii: S0300-9084(19)30165-8.
2018 Lerner MI[1], Mikhaylov G1, Tsukanov AA1, Lozhkomoev AS, Gutmanas E, Gotman I, Bratovs A, Turk V, Turk B, Psakhye SG, Vasiljeva O. Crumpled Aluminum Hydroxide Nanostructures as a Microenvironment Dysregulation Agent for Cancer Treatment. Nano Letters 2018 18(9):5401-5410 (impact factor 12.080).
2014 Mikhaylov G, Klimpel D, Schaschke N, Mikac U, Vizovisek M, Fonovic M, Turk V, Turk B, Vasiljeva O. Selective targeting of tumor and stromal cells by a nanocarrier system displaying lipidated cathepsin B inhibitor. Angewandte Chemie International Editionl. 2014, 53(38):10077-81 (impact factor 11.336).
2014 Maher K, Jerič Kokelj B, Butinar M, Mikhaylov G, Manček-Keber M, Stoka V, Vasiljeva O, Turk B, Grigoryev SA, Kopitar-Jerala N. A role for stefin B (cystatin B) in inflammation and endotoxemia. Journal of Biological Chemistry. 2014, 289(46):31736-50.
2011 Mikhaylov G, Vasiljeva O. Promising approaches in using magnetic nanoparticles in oncology. Biological Chemistry. 2011, 392(11):955-60. Review.
2011 Mikhaylov G, Mikac U, Magaeva AA, Itin VI, Naiden EP, Psakhye I, Babes L, Reinheckel T, Peters C, Zeiser R, Bogyo M, Turk V, Psakhye SG, Turk B, Vasiljeva O. Ferri-liposomes as an MRI-visible drug-delivery system for targeting tumours and their microenvironment. Nature Nanotechnology. 2011, 6(9):594-602 (impact factor 33.265).
2008 Mikhaylov, G, Vasiljeva, O. Technology of the future: the use of magnetic nanoparticles in oncology. The Bulletin of Siberian Branch of Russian Academy of Medical Sciences. 2008, 131(3):17-22. Review.
2008 Milto, I, Mikhaylov, G, Ratkin, A, Magaeva, A. The influence of nanoparticles to morphology of internal organs of mice after intravenous administration of Fe3O4 nanoparticles. The Bulletin of Siberian Medicine. 2008, 1:32-36.
Patents
2018 Schaschke N, Vasiljeva O, Mikhaylov G, Turk B. Cathepsin-binding compound bound to a liposome and its diagnostic and therapeutic use. EP2723387B1. 2018. US (2); EP (2); WO (1).
2014 Vasiljeva O, Itin V, Psakhie S, Mikhaylov G, Mikac M, Turk B, Magaeva A, Naiden E, Terekhova O. Oxide ferrimagnetics with spinel structure nanoparticles and iron oxide nanoparticles, biocompatible aqueous colloidal systems comprising nanoparticles, ferriliposomes, and uses thereof. US20140186268A1. 2014. US (1); EP (1); WO (1).
2013 Psakhie S, Lerner M, Glazkova E, Bakina O, Vasiljeva O, Mikhaylov G, Turk B. Low-Dimensional Structures of Organic and/or Inorganic Substances and Use Thereof. US20160074325A1. 2013. US (1); DE (1); RU (1); WO (1).
2013 Psakhye S, Itin V, Magaeva A, Nayden E, Vasiljeva O, Mikhaylov G, Mikac M, Turk B. Contrast agent for t1 and/or t2 magnetic resonant scanning and method for preparing it. RU2471502C1. 2013. RU (1).

[1] The autors contributed equally to this work

Andreja Kozak

EDUCATION

PhD in nanoscience and Nanotechnology, 2020
Jozef Stefan International Postgraduate School, Ljubljana (Slovenia)
Thesis title: Targeting the tumor microenvironment with nanoparticles for treatment and diagnostics
Mentor: prof. dr. Olga Vasiljeva

M.Sc. in Biochemistry, 2015
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana (Slovenia)
Thesis title: Targeting Cathepsin S with Stefin A-conjugated Liposomes in Inflammation
Mentor: prof. ddr. Boris Turk

B.Sc. in Biochemistry, 2013
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana (Slovenia)
Thesis title: Characterization of Cytotoxicity of Potential Anticancer Drugs on Primary Murine Cells
Mentor: prof. ddr. Boris Turk

RESEARCH EXPERIENCE

01/10/2015–Present: Young researcher
Jozef Stefan Institute, Department of Biochemistry and Molecular Biology
Jamova 39, 1000 Ljubljana (Slovenia)

My research is focused on targeting proteases, namely cathepsins in inflammatory diseases. We prepared a drug delivery system based on stefin A-functionalized liposomes to target cathepsins S and L. We evaluated the system in vitro and in vivo in a murine breast cancer model.
In the second part of my work I study the effect of aluminium-based nanoparticles on tumor cells and their microenvironment.
Additional responsibilities include supervision of undergraduate students.

PUBLICATIONS
    • Bratovs, A., Kramer, L., Mikhaylov, G., Vasiljeva, O., Turk, B. (2019). Stefin A-Functionalized Liposomes As A System For Cathepsins S And L-Targeted Drug Delivery. Biochimie, 166 (2019), 94-102.
    • Lerner, M. I., Mikhaylov, G., Tsukanov, A. A., Lozhkomoev, A. S., Gutmanas, E., Gotman, I., Bratovs, A., Turk, V., Turk, B. Psakhye, S. G., Vasiljeva, O. (2018). Crumpled Aluminum Hydroxide Nanostructures as a Microenvironment Dysregulation Agent for Cancer Treatment. Nano Letters, 18(9), 5401-5410.
CONFERENCES
    • “Stefin A as a ligand for cathepsins S and L-targeted drug delivery”, poster at the European In Vivo Optical Imaging User Group Meeting, Barcelona 2019; Award for most commended poster
    • “A system for cathepsins S and L-targeted drug delivery based on Stefin A-functionalized liposomes”, short talk at the 36th Winter School on Proteinases and Their Inhibitors, Tiers 2019
    • “Stefin A as a ligand for cathepsins S and L-targeted drug delivery”, poster at the 16. International Symposium on Proteases, Inhibitors and Biological Control, Portorož 2018
    • “Targeting cathepsins B, S and L with DARPin/stefinA-conjugated liposomes in cancer”, poster at the Mitochondria, apoptosis and cancer, Bled 2017
TRAINING AND ADDITIONAL SKILLS

Training: Laboratory Animals Training Course, European In Vivo Optical Imaging User Group Meeting 2019 (Workshop)
Languages: Slovene, English, (basics in Italian, Russian, German)
Digital skills: MS Office (Word, Excel, PowerPoint), Adobe Illustrator and Photoshop, basic programming skills (Python)