Georgy Mikhaylov

Researcher & Junior Team Leader (2018–Present)

• Current Research Interests: In vivo imaging, metabolic biochemistry, protease biology, targeted drug delivery for diagnostic and therapeutic applications.

Education:

• PhD in Nanoscience and Nanotechnology
  Jozef Stefan International Postgraduate School, Ljubljana, Slovenia
  September 2014
• M.Sc. in Clinical Biochemistry (Summa Cum Laude)
  Faculty of Medical Biology, Siberian State Medical University, Tomsk, Russia
  June 2007
• M.Sc. in Economy
  Faculty of Business Administration, University of International Business, Almaty, Kazakhstan
  January 2001

Professional Experience:

• Postdoctoral Researcher (2015–2018)
  Laboratory of Bioorganic Chemistry and Molecular Imaging, EPFL, Lausanne, Switzerland
  • Mentor: Tenure Track Assistant Professor Elena Goun (Dubikovskaya)
  • Developed new bioluminescent probes for real-time monitoring of biological processes using biocompatible chemical reactions.
  • Probes demonstrated high selectivity and potential for visualizing metabolic uptake of biological molecules and detecting proteolytic activity in pathological conditions.
  • Research Outputs: Presented at several international conferences and published in Nature Methods (Impact Factor: 28.467).

• Postdoctoral Researcher, Head of Histology Laboratory (2014–2015)
  Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Ljubljana, Slovenia
  • Mentor: Associate Professor Olga Vasiljeva
  • Demonstrated that nanoparticle-based systems capable of disrupting extracellular tumor microenvironment ion balance represent a novel class of inorganic materials with strong antitumor effects.
  • Research Outputs: Published in NanoLetters (Impact Factor: 12.080); contributed to a patent application

• PhD Student & Research Assistant (2008–2014)
  Jozef Stefan International Postgraduate School, Ljubljana, Slovenia
  • Thesis: Development of a Novel Nano-Carriers for Targeted Drug Delivery and Diagnostics
  • Mentor: Associate Professor Olga Vasiljeva
  • Developed new delivery platforms for targeting tumors and their microenvironment, including ferri-liposomes and lipidated inhibitors of cysteine protease (LNC-NS-629).
  • Platforms were shown to act as universal drug delivery systems, successfully delivering various types of cargo to tumor sites.
  • Research Outputs: Presented at international conferences, including XI International Conference on Nanostructured Materials (Rhodes, Greece) and Gordon Research Conference (Barga, Italy). Published in Nature Nanotechnology (Impact Factor: 33.265) and Angewandte Chemie International Edition (Impact Factor: 11.336); contributed to two patent applications.

• Research Associate (2007–2008)
  Department of Biochemistry and Molecular and Structural Biology, Jozef Stefan Institute, Ljubljana, Slovenia
  • Demonstrated the potential use of Fe3O4 nanoparticles as MRI-visible contrast agents for cancer diagnostics.
  • Research Outputs: Presented at an international conference and published a review article

• Master Student (2001–2007)
  Department of Human Morphology, Siberian State Medical University, Tomsk, Russia
  • Thesis: An Assessment of Acute Toxicity of Fe3O4 Nanoparticles in Rats
  • Mentor: Professor Irina Sukhodolo
  • Evaluated the possible adverse effects of Fe3O4 nanoparticles in rats; findings showed non-toxicity in high doses, suggesting potential use in targeted drug delivery systems.
  • Research Outputs: Resulted in one publication.

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Key Publications:

• Maric T, Mikhaylov G, et al. (2019). Bioluminescent-based imaging and quantification of glucose uptake in vivo. Nature Methods, 16(6), 526-532.
• Bratos A, Kramer L, Mikhaylov G, et al. (2019). Stefin A-functionalized liposomes as a system for cathepsin S and L-targeted drug delivery. Biochimie, pii: S0300-9084(19)30165-8.
• Lerner MI, Mikhaylov G, et al. (2018). Crumpled Aluminum Hydroxide Nanostructures as a Microenvironment Dysregulation Agent for Cancer Treatment. Nano Letters, 18(9), 5401-5410.
• Mikhaylov G, et al. (2014). Selective targeting of tumor and stromal cells by a nanocarrier system displaying lipidated cathepsin B inhibitor. Angewandte Chemie International Edition, 53(38), 10077-81.
• Maher K, Jerič Kokelj B, Butinar M, Mikhaylov G, et al. (2014). A role for stefin B (cystatin B) in inflammation and endotoxemia. Journal of Biological Chemistry, 289(46), 31736-50.
• Mikhaylov G, Vasiljeva O. (2011). Promising approaches in using magnetic nanoparticles in oncology. Biological Chemistry, 392(11), 955-60.
• Mikhaylov G, et al. (2011). Ferri-liposomes as an MRI-visible drug-delivery system for targeting tumours and their microenvironment. Nature Nanotechnology, 6(9), 594-602.
• Mikhaylov, G, Vasiljeva, O. (2008). Technology of the future: the use of magnetic nanoparticles in oncology. The Bulletin of Siberian Branch of Russian Academy of Medical Sciences, 131(3), 17-22.
• Milto, I, Mikhaylov, G, et al. (2008). The influence of nanoparticles to morphology of internal organs of mice after intravenous administration of Fe3O4 nanoparticles. The Bulletin of Siberian Medicine, 1:32-36.

Patents:

• Schaschke N, Vasiljeva O, Mikhaylov G, et al. (2018). Cathepsin-binding compound bound to a liposome and its diagnostic and therapeutic use. EP2723387B1.
• Vasiljeva O, Itin V, Psakhie S, Mikhaylov G, et al. (2014). Oxide ferrimagnetics with spinel structure nanoparticles and biocompatible aqueous colloidal systems. US20140186268A1.
• Psakhie S, Lerner M, Glazkova E, Bakina O, Vasiljeva O, Mikhaylov G, et al. (2013). Low-Dimensional Structures of Organic and/or Inorganic Substances and Use Thereof. US20160074325A1.
• Psakhye S, Itin V, Magaeva A, Nayden E, Vasiljeva O, Mikhaylov G, et al. (2013). Contrast agent for T1 and/or T2 magnetic resonance scanning and method for preparing it. RU2471502C1.

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