Katarina Karničar

PROFESSIONAL SUMMARY

I am a postdoctoral research fellow in the Structural biology group. My main project is focused on a multi-protein complex, major histocompatibility complex class II molecules in complex with invariant chain (MHCII-Ii complex). By combining different techniques, we are trying to determine the 3D structure of the MHCII-Ii complex.

CORE QUALIFICATIONS
      • Production of recombinant proteins using different expression systems (bacterial, yeast, insect).
      • Protein isolation and purification using FPLC systems (affinity, size-exclusion, ion-exchange chromatography)
      • Protein separation and detection techniques
      • Molecular cloning (classical cloning using restriction and ligation, ligation independent cloning)
EXPERIENCE

2016 – Postdoctoral Researcher   Jožef Stefan Institute, The Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins (CIPkebip)

2009 – 2015 Research Fellow   ACIES BIO LTD.

2008 – 2009 Researcher   Biotechnical Faculty

Memberships: Slovenian Biochemical Society

EDUCATION

2009  – 2015 PhD in Biotechnology (Doctoral programme of Biosciences, Field Biotechnology, Biotechnical Faculty, University of Ljubljana, Slovenia)

2001 – 2007 BSc in Microbiology (Interdepartmental programme of Microbiology, Biotechnical Faculty, University of Ljubljana, Slovenia)

Courses and conference attendances:
      • September 2019: 13th Meeting of the Slovenian Biochemical Society with International Participation, Dobrna, Slovenia
      • March 2018: Radiation Safety Short Course at Milan Čopič Nuclear Training Centre (ICJT), Podgorica, Slovenia
      • November 2013: Conference KC Brin: Food and health – trends and challenges
      • September 2013: 10. Meeting of the Slovenian Biochemical Society
      • September 2011: Conference CIPKEBIP: Biosynthesis and metabolic engineering in development of drugs and bioprocesses in industry
      • December 2009: BIA and biotechnology in Slovenian developmental path, Expert Meeting on the 20th anniversary of the company
      • January 2009: 6. Annual conference of Chair of biotechnology, microbiology and food safety: Antimicrobial substances in microbiology and biotechnology
      • January 2008: 5. Annual conference of Chair of biotechnology, microbiology and food safety: Biotechnology and microbiology in the future: Proteomics
BIBLIOGRAPHY

Original research papers:

KARNIČAR, Katarina, DROBNAK, Igor, PETEK, Marko, MAGDEVSKA, Vasilka, HORVAT, Jaka, VIDMAR, Robert, BAEBLER, Špela, ROTTER, Ana, JAMNIK, Polona, FUJS, Štefan, TURK, Boris, FONOVIĆ, Marko, GRUDEN, Kristina, KOSEC, Gregor, PETKOVIĆ, Hrvoje. Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraea. Microbial cell factories. 2016, vol. 15, no. 93, p. 1/17-17/17, ilustr. ISSN 1475-2859. http://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-016-0496-5, DOI: 10.1186/s12934-016-0496-5. [COBISS.SI-ID 4655992]

KIRM, Benjamin, MAGDEVSKA, Vasilka, TOME, Miha, HORVAT, Marinka, KARNIČAR, Katarina, PETEK, Marko, VIDMAR, Robert, BAEBLER, Špela, JAMNIK, Polona, FUJS, Štefan, HORVAT, Jaka, FONOVIĆ, Marko, TURK, Boris, GRUDEN, Kristina, PETKOVIĆ, Hrvoje, KOSEC, Gregor. SACE_5599, a putative regulatory protein, is involved in morphological differentiation and erythromycin production in Saccharopolyspora erythraea. Microbial cell factories. 2013, vol. 12, p. 126-1-126-15. ISSN 1475-2859. http://www.microbialcellfactories.com/content/12/1/126/abstract, DOI: 10.1186/1475-2859-12-126. [COBISS.SI-ID 3005775]

 

Conference contributions:

KARNIČAR, Katarina, TALER-VERČIČ, Ajda, USENIK, Aleksandra, LINDIČ, Nataša, PINTAR, Sara, LOBODA, Jure, SEKIRNIK, Andreja, TUŠAR, Livija, FONOVIĆ, Marko, VIDMAR, Robert, TURK, Dušan. A platform for protein production and crystal structure determination. V: KISOVEC, Matic (ur.). Book of abstracts = Knjiga povzetkov. 13th Meeting of the Slovenian Biochemical Society with International Participation, Dobrna 24-27 September 2019. Ljubljana: Slovenian Biochemical Society, 2019. Str. 71. ISBN 978-961-93879-8-6. [COBISS.SI-ID 32879655]

TALER-VERČIČ, Ajda, USENIK, Aleksandra, LINDIČ, Nataša, KARNIČAR, Katarina, PINTAR, Sara, LOBODA, Jure, ARNOLJ, Marjeta, PRETNAR, Gregor, TOMAŽ, Špela, TURK, Dušan. From genes to protein crystals : platform for high throughput protein production and structure determination. V: GORIČAR, Katja (ur.), HUDLER, Petra (ur.). Book of abstracts = Zbornik povzetkov. 12th Meeting of the Slovenian Biochemical Society with International Participation = 12. srečanje Slovenskega biokemijskega društva z mednarodno udeležbo, Bled 20 – 23 september 2017. Ljubljana: Slovenian Biochemical Society, 2017. Str. 121. ISBN 978-961-93879-4-8. [COBISS.SI-ID 31101991]

PETKOVIĆ, Hrvoje, MAGDEVSKA, Vasilka, KIRM, Benjamin, TOME, Miha, HORVAT, Marinka, KARNIČAR, Katarina, PETEK, Marko, VIDMAR, Robert, BAEBLER, Špela, JAMNIK, Polona, FUJS, Štefan, HORVAT, Jaka, FONOVIĆ, Marko, TURK, Boris, GRUDEN, Kristina, KOSEC, Gregor. Identifikacija domnevnega regulatornega proteina, ki je udeležen pri biosintezi eritromicina in diferenciaciji Saccharopolyspora erythraea, z omskimi pristopi. V: KUŠAR, Darja (ur.), OCEPEK, Matjaž (ur.). Knjiga povzetkov : kongres SMD 2014. 6. kongres Slovenskega mikrobiološkega društva, 24.-26. september 2014, Bled, Slovenija. Ljubljana: Veterinarska fakulteta, 2014. Str. 60, pr-21. ISBN 978-961-6199-67-4. [COBISS.SI-ID 3211343]